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Background: Vibratory massage is now widely used to alleviate muscle fatigue. The effects of different vibration massage intensities on left and right upper trapezius (UT) fatigue have not been examined. Therefore, the present study first examined whether a vibration massage intervention had an effect on UT muscles and second compared the effects of 2 different levels (36 Hz and 46 Hz) of vibratory massage on the right and left oblique muscles under 3 different fatigue conditions. Methods: A total of 23 participants (12 female, 11 male; age: 26.5 ± 3.9 year, height: 170.5 ± 1.6 cm, mass: 57.5 ± 1.5 kg, BMI: 24.3 ± 1.6 kg/m2) were randomly divided into intervention and control groups. The 2 groups of subjects completed isometric contraction fatigue tasks of 30 s of fatigue, 60 s of fatigue and 90 s of fatigue in turn. The specific task of isotonic contraction was performed by subjects holding a 1 kg dumbbell in each hand and performing a straight arm weighted lateral supination exercise. After each exercise, the intervention group was randomized to apply a massage device with a vibration intensity of 36 Hz or 46 Hz on the left and right UT muscles for 5 min each. The control group did not receive any treatment. Both groups then repeated the same fatigue task as before. Then, the effects of different vibration massage interventions on UT muscles were derived by analyzing the changes in maximal voluntary contraction percentage (MVC%) of surface electromyography (sEMG) signals before and after the intervention, and the most effective vibration massage program for relieving left and right UT fatigue was summarized. Then, four classification algorithms were used to label and classify the collected sEMG data, and finally a UT muscle fatigue identification and vibration massage model was constructed. Results: After using the vibration massage level 1 (36 Hz) intervention, the MVC% of the right UT muscle showed significant reductions in the 30 s fatigue task, the 60 s fatigue task and the 90 s fatigue task (R1: p = 0.022, R2: p = 0.005, R3: p = 0.049). After using the vibration massage level 3 (46 Hz) intervention, the MVC% of the right UT muscle showed a significant decrease in both the 60 s fatigue task and the 90 s fatigue task (R2: p = 0.033, R3: p = 0.028). Significant decreases in MVC% for the left UT muscle were found only in the 90 s fatigue task (L3: p = 0.040). Then, by comparing the different performances of four commonly used classification algorithms, it was found that the bagging (accuracy = 0.860) algorithm had higher accuracy. Therefore, the bagging algorithm was used for the UT fatigue identification and vibration massage models. Conclusions: This was the first study to show the impacts of different levels of vibration massage on fatigue alleviation in the left and right UT muscles. Furthermore, the bilateral UT fatigue identification and vibration massage model developed in this study can help people to choose the most appropriate massage protocol for quick relief and relaxation of the UT muscles under three different fatigue tasks.

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